Table 2 Primer sequences.
From: Generation of proliferating human adult hepatocytes using optimized 3D culture conditions
Gene | Forward primer | Reverse primer |
|---|---|---|
GAPDH | GTGGACCTGACCTGCCGTCT | GGAGGAGTGGGTGTCGCTGT |
E-CADHERIN | TGCTCTTGCTGTTTCTTCGG | TGCCCCATTCGTTCAAGTAG |
N-CADHERIN | GTGCATGAAGGACAGCCTCT | ATGCCATCTTCATCCACCTT |
a-FETOPROTEIN | TGCAGCCAAAGTGAAGAGGGAAGA | CATAGCGAGCAGCCCAAAGAAGAA |
ALBUMIN | GGGCATGTTTTTGTATGAAT | CCCACTTTTCCTAGGTTTCT |
HNF4a | CAGAAGGCACCAACCTCAAC | CTCGAGGCACCGTAGTGTTT |
HNF1β | GCCTTAGTGGAGGAATGCAA | GAGGGTTCAGGCTGTGAGTC |
UGT1A1 | TGACGCCTCGTTGTACATCAG | CCTCCCTTTGGAATGGCAC |
NAT1 | ACCATTGATGGCAGGAACTA | TGTTCCCTTCTGATTTGGTC |
GSTA1/2 | CGCTACTTCCCTGCCTTTGA | AGTCAAGCTCCTCGACGTAG |
MRP2 | TGAGCAAGTTTGAAACGCACAT | AGCTCTTCTCCTGCCGTCTCT |
OCT1 | TAATGGACCACATCGCTCAA | AGCCCCTGATAGAGCACAGA |
BSEP | TGATCCTGATCAAGGGAAGG | TGGTTCCTGGGAAACAATTC |
CAR | TGATCAGCTGCAAGAGGAGA | AGGCCTAGCAACTTCGCATA |
PXR | CCAGGACATACACCCCTTTG | CTACCTGTGATGCCGAACAA |
AhR | CTTCCAAGCGGCATAGAGAC | AGTTATCCTGGCCTCCGTTT |
