Figure 4

Abundance (calculated as RPM) of crAssphage and Bacteroides intestinalis. Libraries of cat feces pools were generated by random RT-PCR amplification of VLP nucleic acids. For the case cat, total fecal supernatant nucleic acids were extracted without filtration and nuclease treatment steps and processed using RCA. Illumina libraries were then generated using Nextera XT and sequenced on a MiSeq. Bowtie was used to then quantify reads of crAssphage and Bacteroides intestinalis.