Figure 2 | Scientific Reports

Figure 2

From: The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome

Figure 2

Live cell Ca2+ imaging in hDPSCs. (A, B) show representative time courses of gross intracellular Ca2+ dynamics for Biodentine (A) and ProRoot WMTA (B) induced by the two biomaterials (averaged signal over all cells in view). Arrows indicate exposure to the biomaterial lowered to contact the recording medium. (C,E,G) show representative Ca2+ images for Biodentine, with (C) representing a B/W image of stem cells loaded with fluorescent Ca2+ indicator to reveal the cells, (E) a pseudocolored Ca2+ image taken before biomaterial exposure and (G) a Ca2+ image at the peak of the global Ca2+ change. (D,F,H) show corresponding images for exposure to ProRoot WMTA. Scale bar measures 50 µm; pseudocolor scale indicates fluorescent Ca2+ indicator signal level.

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