Figure 2

Expression type composition of ICM spheroids for H₁. (a) ICM organoids (experimental data) for 24 h and 48 h and simulated ICM spheroids for \(t_1\) and \(t_2\). (b) Expression type composition of ICM organoids and ICM spheroids as percentage of the total number of cells within ICM organoids at \(t_1\) and \(t_2\). Simulations were performed under the assumption H₁. Experimental data from Mathew et al. are indicated by triangles. Simulation results for different \(t_0\) are indicated by circles. The error bars indicate the standard deviation. \(t_0\) from lowest line to top: 200, 300 and 400 cells. Statistically significant differences between the cell fate proportion of ICM organoids and ICM spheroids are indicated by stars (\(p < 0.05\); using a Wilcoxon–Mann–Whitney test with Bonferroni correction). (c) The effect size (\(\psi\)) as the relative deviation of the simulated and experimental neighbourhood statistics at \(t_1\) for simulations performed under the assumptions H₁, H₂, H₃ and H₄.