Figure 3

T cell activation induces genome-wide changes in gene-regulatory interactions. (A) Barcode enrichment plot showing that activation-associated DIs in CD4⁺ T cells are similarly ranked in CD8⁺ T cells. The plot is exactly analogous to Figs. 2C,D but for DI bin-pairs instead of DA peaks or DE genes. (B) Box plots showing that the increased accessibility at DA regions in gene-regulatory chromatin interactions ‘gained’ is higher than that at gene-regulatory chromatin interactions ‘unchanged’ or ‘lost’, following CD4⁺ (left) and CD8⁺ (right) T cell activation. Shown are the median (central horizontal line), interquartile range (boxes), values of the upper and lower quartiles (whiskers), outliers beyond 1.5 IQR (circles). Statistical comparisons were made with the unpaired Wilcoxon test. (C) Pie chart showing distribution of down-regulated, up-regulated or unchanged target genes linked to ‘lost’ (top) or ‘gained’ (bottom) of regulatory interactions..