Table 3 Effect of in-vitro exposure of spermatozoa to different concentrations of mercuric chloride (0.031–1.25 µg/ml) on motile spermatozoa count at different time intervals.

From: Collapsed mitochondrial cristae in goat spermatozoa due to mercury result in lethality and compromised motility along with altered kinematic patterns

 

Motility

Rapid progressive

Slow progressive

Non-progressive

VCL

VAP

VSL

LIN%

STR%

WOB%

BCF

maxALH

15 min

Control

85.94 ± 1.2a

74.98 ± 0.16a

2.23 ± 0.33a

6.72 ± 1.45a

161.69 ± 0.23a

129.26 ± 0.17a

123.6 ± 0.61a

77.44 ± 0.53a

95.62 ± 1.36ab

80.74 ± 0.45

41.27 ± 0.31a

3.8 ± 0.28a

A

88.50 ± 1.2a

78.39 ± 1.26a

2.96 ± 0.27a

6.96 ± 1.23a

160.08 ± 0.51a

128.21 ± 0.48a

121.3 ± 0.42a

78.46 ± 1.30a

94.61 ± 1.32ab

81.51 ± 0.82

40.26 ± 0.44a

3.7 ± 0.22a

B

86.95 ± 0.5a

78.18 ± 1.22a

2.82 ± 0.61a

4.08 ± 1.7a

159.32 ± 0.61a

125.18 ± 1.29ab

120.02 ± 1.03a

77.21 ± 1.40a

93.68 ± 1.44a

81.42 ± 1.48

39.67 ± 1.11ab

3.51 ± 0.41a

C

85.62 ± 0.6a

76.20 ± 0.72a

3.40 ± 0.39a

6.48 ± 1.02a

156.25 ± 1.71ab

122.9 ± 0.36b

117.52 ± 1.20a

76.28 ± 1.58a

94.04 ± 1.03ab

82.14 ± 1.20

37.28 ± 0.49ab

3.3 ± 0.20a

D

84.75 ± 0.3ab

74.42 ± 0.81a

2.48 ± 0.61a

6.44 ± 1.31a

150.11 ± 1.43bc

120.1 ± 0.81b

111.17 ± 1.25b

78.01 ± 1.20a

95.07 ± 1.05ab

82.74 ± 1.06

34.25 ± 0.55b

2.91 ± 0.11ab

1 h

Control

84.47 ± 1.7a

71.53 ± 0.27ab

2.22 ± 0.29a

7.47 ± 1.03a

160 ± 0.38a

129.03 ± 1.49a

123.03 ± 1.04a

77.275 ± 1.06a

95.34 ± 1.30ab

80.64 ± 1.72

40.52 ± 0.31a

3.8 ± 0.21a

A

78.06 ± 0.3ab

68.02 ± 0.41b

4.21 ± 1.37ab

6.24 ± 1.36a

158.13 ± 1.45a

125.1 ± 1.48a

118.27 ± 0.36a

78.76 ± 0.91a

94.34 ± 1.20ab

81.47 ± 1.25

39.01 ± 0.32ab

3.2 ± 0.22a

B

77.73 ± 1.2b

67.55 ± 1.59b

7.36 ± 1.82ab

7.81 ± 1.05a

152.45 ± 1.62b

122.11 ± 1.72b

113.31 ± 0.47b

79.58 ± 1.22a

93.33 ± 1.03a

80.53 ± 1.34

39.08 ± 0.33ab

2.81 ± 0.10a

C

72.73 ± 1.0b

65.04 ± 0.61b

5.23 ± 2.21ab

8.18 ± 1.51a

150.94 ± 0.91bc

119.42 ± 1.04b

106.16 ± 0.62c

80.03 ± 1.42a

94.7 ± 1.20ab

82.04 ± 1.44

36.28 ± 0.29ab

2.52 ± 0.31ab

D

65.79 ± 0.4c

58.78 ± 0.39c

2.38 ± 1.40a

9.20 ± 0.91a

148.7 ± 0.79bc

116.08 ± 1.81c

99.32 ± 0.82d

80.21 ± 1.39a

95.37 ± 1.25ab

83.06 ± 1.53

33.28 ± 0.71b

2.4 ± 0.13ab

3 h

Control

83.61 ± 0.12a

70.57 ± 0.18ab

4.46 ± 0.41ab

8.54 ± 1.61a

159.14 ± 1.28a

128.31 ± 0.15a

122.17 ± 0.41a

76.06 ± 1.04a

95.12 ± 1.7ab

80.27 ± 0.88

39.74 ± 0.48ab

3.7 ± 0.29a

A

68.23 ± 1.16c

52.29 ± 1.26c

9.86 ± 1.6b

10.78 ± 1.29a

145.28 ± 1.32c

120.17 ± 1.51b

114.07 ± 1.48b

77.03 ± 1.42a

97.3 ± 1.5ab

82.46 ± 0.94

36.04 ± 0.29ab

2.8 ± 0.21a

B

63.56 ± 1.11c

39.02 ± 1.51d

20.59 ± 0.26c

19.07 ± 1.36b

132.02 ± 2.01d

110.28 ± 1.04d

107.06 ± 1.61c

78.85 ± 0.81a

97.19 ± 1.07ab

82.53 ± 1.37

35.24 ± 0.71b

2.4 ± 0.28ab

C

47.78 ± 0.31d

28.49 ± 1.21e

24.67 ± 1.30 cd

28.55 ± 1.37c

128.17 ± 1.41d

106.02 ± 1.01d

95.06 ± 1.03d

80.92 ± 1.48a

98.62 ± 1.40b

83.41 ± 1.40

27.93 ± 0.66c

2 ± 0.41ab

D

39.84 ± 0.28e

19.9 ± 0.82f.

29.62 ± 1.7d

41.31 ± 1.49d

120.27 ± 0.27e

97.27 ± 1.06e

84.25 ± 0.62e

81.19 ± 1.71a

98.51 ± 1.03b

84.37 ± 1.29

20.61 ± 0.10d

1.7 ± 0.66

p-value

0.005

0.0001

0.0123

0.0432

0.0031

0.004

0.022

0.714

0.041

0.844

0.041

0.022

  1. Data presented are mean ± SE of the semen samples of six bucks.
  2. VCL curvilinear velocity (µm s−1), VSL straight-line velocity (µm s−1), VAP average path velocity (µm s−1), LIN linearity of forward progression (%), STR straightness (%), WOB wobble (%), maxALH maximum amplitude of lateral head displacement (µm), BCF beat-cross frequency (Hz).
  3. A = 0.031 µg/ml, B = 0.125 µg/ml, C = 0.25 µg/ml, and D = 1.25 µg/ml HgCl2.
  4. Different small superscripts in the columns indicate significant (p < 0.05) differences in kinematic patterns between control and different treatment groups at different time intervals.