Table 3 Effect of in-vitro exposure of spermatozoa to different concentrations of mercuric chloride (0.031–1.25 µg/ml) on motile spermatozoa count at different time intervals.
Motility | Rapid progressive | Slow progressive | Non-progressive | VCL | VAP | VSL | LIN% | STR% | WOB% | BCF | maxALH | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
15 min | ||||||||||||
Control | 85.94 ± 1.2a | 74.98 ± 0.16a | 2.23 ± 0.33a | 6.72 ± 1.45a | 161.69 ± 0.23a | 129.26 ± 0.17a | 123.6 ± 0.61a | 77.44 ± 0.53a | 95.62 ± 1.36ab | 80.74 ± 0.45 | 41.27 ± 0.31a | 3.8 ± 0.28a |
A | 88.50 ± 1.2a | 78.39 ± 1.26a | 2.96 ± 0.27a | 6.96 ± 1.23a | 160.08 ± 0.51a | 128.21 ± 0.48a | 121.3 ± 0.42a | 78.46 ± 1.30a | 94.61 ± 1.32ab | 81.51 ± 0.82 | 40.26 ± 0.44a | 3.7 ± 0.22a |
B | 86.95 ± 0.5a | 78.18 ± 1.22a | 2.82 ± 0.61a | 4.08 ± 1.7a | 159.32 ± 0.61a | 125.18 ± 1.29ab | 120.02 ± 1.03a | 77.21 ± 1.40a | 93.68 ± 1.44a | 81.42 ± 1.48 | 39.67 ± 1.11ab | 3.51 ± 0.41a |
C | 85.62 ± 0.6a | 76.20 ± 0.72a | 3.40 ± 0.39a | 6.48 ± 1.02a | 156.25 ± 1.71ab | 122.9 ± 0.36b | 117.52 ± 1.20a | 76.28 ± 1.58a | 94.04 ± 1.03ab | 82.14 ± 1.20 | 37.28 ± 0.49ab | 3.3 ± 0.20a |
D | 84.75 ± 0.3ab | 74.42 ± 0.81a | 2.48 ± 0.61a | 6.44 ± 1.31a | 150.11 ± 1.43bc | 120.1 ± 0.81b | 111.17 ± 1.25b | 78.01 ± 1.20a | 95.07 ± 1.05ab | 82.74 ± 1.06 | 34.25 ± 0.55b | 2.91 ± 0.11ab |
1 h | ||||||||||||
Control | 84.47 ± 1.7a | 71.53 ± 0.27ab | 2.22 ± 0.29a | 7.47 ± 1.03a | 160 ± 0.38a | 129.03 ± 1.49a | 123.03 ± 1.04a | 77.275 ± 1.06a | 95.34 ± 1.30ab | 80.64 ± 1.72 | 40.52 ± 0.31a | 3.8 ± 0.21a |
A | 78.06 ± 0.3ab | 68.02 ± 0.41b | 4.21 ± 1.37ab | 6.24 ± 1.36a | 158.13 ± 1.45a | 125.1 ± 1.48a | 118.27 ± 0.36a | 78.76 ± 0.91a | 94.34 ± 1.20ab | 81.47 ± 1.25 | 39.01 ± 0.32ab | 3.2 ± 0.22a |
B | 77.73 ± 1.2b | 67.55 ± 1.59b | 7.36 ± 1.82ab | 7.81 ± 1.05a | 152.45 ± 1.62b | 122.11 ± 1.72b | 113.31 ± 0.47b | 79.58 ± 1.22a | 93.33 ± 1.03a | 80.53 ± 1.34 | 39.08 ± 0.33ab | 2.81 ± 0.10a |
C | 72.73 ± 1.0b | 65.04 ± 0.61b | 5.23 ± 2.21ab | 8.18 ± 1.51a | 150.94 ± 0.91bc | 119.42 ± 1.04b | 106.16 ± 0.62c | 80.03 ± 1.42a | 94.7 ± 1.20ab | 82.04 ± 1.44 | 36.28 ± 0.29ab | 2.52 ± 0.31ab |
D | 65.79 ± 0.4c | 58.78 ± 0.39c | 2.38 ± 1.40a | 9.20 ± 0.91a | 148.7 ± 0.79bc | 116.08 ± 1.81c | 99.32 ± 0.82d | 80.21 ± 1.39a | 95.37 ± 1.25ab | 83.06 ± 1.53 | 33.28 ± 0.71b | 2.4 ± 0.13ab |
3 h | ||||||||||||
Control | 83.61 ± 0.12a | 70.57 ± 0.18ab | 4.46 ± 0.41ab | 8.54 ± 1.61a | 159.14 ± 1.28a | 128.31 ± 0.15a | 122.17 ± 0.41a | 76.06 ± 1.04a | 95.12 ± 1.7ab | 80.27 ± 0.88 | 39.74 ± 0.48ab | 3.7 ± 0.29a |
A | 68.23 ± 1.16c | 52.29 ± 1.26c | 9.86 ± 1.6b | 10.78 ± 1.29a | 145.28 ± 1.32c | 120.17 ± 1.51b | 114.07 ± 1.48b | 77.03 ± 1.42a | 97.3 ± 1.5ab | 82.46 ± 0.94 | 36.04 ± 0.29ab | 2.8 ± 0.21a |
B | 63.56 ± 1.11c | 39.02 ± 1.51d | 20.59 ± 0.26c | 19.07 ± 1.36b | 132.02 ± 2.01d | 110.28 ± 1.04d | 107.06 ± 1.61c | 78.85 ± 0.81a | 97.19 ± 1.07ab | 82.53 ± 1.37 | 35.24 ± 0.71b | 2.4 ± 0.28ab |
C | 47.78 ± 0.31d | 28.49 ± 1.21e | 24.67 ± 1.30 cd | 28.55 ± 1.37c | 128.17 ± 1.41d | 106.02 ± 1.01d | 95.06 ± 1.03d | 80.92 ± 1.48a | 98.62 ± 1.40b | 83.41 ± 1.40 | 27.93 ± 0.66c | 2 ± 0.41ab |
D | 39.84 ± 0.28e | 19.9 ± 0.82f. | 29.62 ± 1.7d | 41.31 ± 1.49d | 120.27 ± 0.27e | 97.27 ± 1.06e | 84.25 ± 0.62e | 81.19 ± 1.71a | 98.51 ± 1.03b | 84.37 ± 1.29 | 20.61 ± 0.10d | 1.7 ± 0.66 |
p-value | 0.005 | 0.0001 | 0.0123 | 0.0432 | 0.0031 | 0.004 | 0.022 | 0.714 | 0.041 | 0.844 | 0.041 | 0.022 |