Table 5 Effect of in-vitro exposure of buck-spermatozoa to different concentrations of mercuric chloride (0.031–1.25 µg/ml) on early apoptosis and necrosis at different time intervals.

Treatments	% Apoptotic and necrotic spermatozoa at different time intervals
	15 min		3 h
	Necrosis	Apoptosis	Necrosis	Apoptosis
Positive control*	5.00 ± 0.58	29.67 ± 0.33	8.33 ± 0.88	41.67 ± 2.40
PBS Control	6.00 ± 1.53^D	0.00 ± 0.00	11.00 ± 2.65^D	0.67 ± 0.00
0.031 µg/ml Mercuric chloride	10.00 ± 1.15^C	0.00 ± 0.00	18.31 ± 2.85^CD	0.00 ± 0.00
0.125 µg/ml Mercuric chloride	14.67 ± 0.33^B	0.00 ± 0.00	23.45 ± 3.38^BC	0.00 ± 0.00
0.25 µg/ml Mercuric chloride	18.68 ± 0.33^AB	0.00 ± 0.00	32.13 ± 3.38^AB	0.00 ± 0.00
1.25 µg/ml Mercuric chloride	22.00 ± 0.58^A	0.00 ± 0.00	39.00 ± 2.65^A	0.00 ± 0.00
p-value	0.000	0.0	0.000	0.0

Data presented are mean ± SE of the semen samples of six bucks.
Different capital superscripts in the columns indicate significant (p < 0.05) differences between the different treatment groups.
*Camptothecin (10 µM) treated buck semen sample.

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