Figure 1 | Scientific Reports

Figure 1

From: The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region

Figure 1

Identification of the cytosolic oligomerization site. (A) Schematic representation of Mouse LJaw1 (LJaw1; long form Jaw1) and mutants. N-term; N-terminal region, coiled-coil; coiled-coil domain, Stem; stem region, Gray box; single trans-membrane domain. (B) Identification of the oligomerization site by co-immunoprecipitation followed by western blotting. FLAG Ms LJaw1 was co-expressed with GFP, GFP Ms LJaw1 or mutants in HEK293 cells by transfection. After incubation for 24 h, the lysates were subjected to co-immunoprecipitation. For western blotting, an anti-FLAG rabbit antibody and an anti-GFP rabbit antibody as primary antibodies were used. (C) GFP, GFP Ms LJaw1 or mutants was expressed alone or co-expressed with FLAG Ms LJaw1 in B16F10 cells by transfection. After incubation for 24 h, the cells were subjected to immunostaining using an anti-FLAG rabbit antibody as a primary antibody. The images were acquired by confocal microscopy. Scale bar; 20 μm.

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