Figure 4
From: ROCK inhibition reduces morphological and functional damage to rod synapses after retinal injury
Morphological results 2 days after retinal detachment. (A) Detachments have spontaneously reattached (red arrows). (B) Retraction in the saline-treated eye detached area (BD) and the drug-treated eye, detached area (AD). Retina labeled for synaptic vesicle protein (SV2, green) and nuclei (propidium iodide, PI, red). (C) Axonal retraction was significantly reduced by 29.7% in AR13503-treated eyes compared to the eyes that received BSS alone (n = 6 animals, *p = 0.04, using 60 images/eye, + /- SD). Attached and detached areas of each eye were combined for this analysis (AC + AD vs. BC + BD). Data on the individual areas are reported in the Results. (D) Sprouting of bipolar cells. Retinae labeled for synaptic vesicle protein (SV2, red) and rod bipolar cells (anti-protein kinase C-alpha, PKC-alpha, green). White arrows indicate the fine dendritic processes of the bipolar cells extending into the outer nuclear layer (ONL). Pictures were taken of detached-spontaneously reattached areas both from BSS- (BD) and drug-treated (AD) eyes.
