Figure 5

Subcellular localization of SaAACT and SaHMGS proteins in A. thaliana mesophyll protoplasts. Yellow fluorescence indicates SaAACT-YFP and SaHMGS-YFP fusion protein signal. Blue signal indicates chlorophyll (Chl) autofluorescence and red signal indicates m-Cherry fluorescence. The merged images represent a digital combination of Chl autofluorescence, YFP fluorescent and m-Cherry protein fluorescence images. Fluorescence was excited for YFP at 514 nm, for Chl at 543 nm and for m-Cherry at 587 nm. Scale bar of SaHMGS-YFP and YFP = 5 μm and scale bar of SaAACT-YFP = 8 μm.