Table 3 Specific primers used in this study.
Primer purpose | Primer name | Primer sequence (5′ → 3′) |
|---|---|---|
5′ RACE | AACT-5(1)/(2) | CACAAACACCCATGCCAAAATCA/TGCTTTCCATGCCACCAGCTACA |
HMGS-5(1)/(2) | AGTCTCGCTGCCTACTTCCATCC/TCCAGTGCTTCCTGTTGAACACA | |
3′ RACE | AACT-3(1)/(2) | GAGGAAGCTCCGACCAAGTTTTA/GGAGCTGTATCTCTGGGACATC |
HMGS-3(1)/(2) | CTCAGTCAGCATGCCTAAACCT/CATTGTTGCCGGCTCTGTTC | |
ORF | AACT-O(F)/(R) | ATGGCTCCATCCGGGACGAAAGC/TAGCTTTGAAGGTCCTACTCTCA |
HMGS-O(F)/(R) | ATGGCTTCGCAGCAGAAGAAT/TTAATGGCCATTGGGAATTGAACCA | |
qRT-PCR | q-AACT-F/R | CGGGACGAAAGCCTCTTATT/AGGGAACCAAGAAAGTCACC |
q-HMGS-F/R | TCTCGCCATTGATGTCTACTTT/CCCAATGGTGTACTTCCCTTTA | |
Functional complementation | pYES2-AACT-F | CAGTGTGCTGGAATTCATGGCTCCATCCGGGACG |
pYES2-AACT-R | CATGCTCGAGCGGCCGCTAGCTTTGAAGGTCCTACTCTCAAG | |
pYES2-HMGS-F | CAGTGTGCTGGAATTCATGGCTTCGCAGCAGAAGA | |
pYES2-HMGS-R | CATGCTCGAGCGGCCGCATGGCCATTGGGAATTGAACCA | |
Subcellular localization | YFP-AACT-F | CCGGAATTCATGGCTCCATCCGGGACGAAAGC |
YFP-AACT-R | CGCGGATCCTAGCTTTGAAGGTCCTACTCTCA | |
YFP-HMGS-F | CCGGAATTCATGGCTTCGCAGCAGAAGAA | |
YFP-HMGS-R | CGCGGATCCATGGCCATTGGGAATTGAACCA |
