Figure 6

The expression levels of ApCDA and ApKNK at 24 h and 48 h after RNAi of ApTPS and ApTRE. (A) Chitin deacetylase gene, (B) Chitin-binding protein Knickkopf gene, (C, D) The correlation analyses between the trehalase activity and the expression of ApTRE. Each point in the figure represents a sample, and the P-values and the correlation coefficient r are presented. Bivariate correlations between variables were calculated using Spearman’s rank correlation coefficients. CK, the normal diet; dsGFP, the GFP-dsRNA treatment; dsTPS, the TPS-dsRNA treatment; dsTRE, the TRE-dsRNA treatment. Each bar represents the Means ± SEM from three biological replicates and three technical replicates and the data were analyzed using one-way analysis of variance (ANOVA), followed by the Tukey–Kramer test. The mRNA expression level in the artificial diet group was designated as control, different letters above the error bars indicate significant differences (P < 0.05).