Figure 2

Comparison of performance of 15 RT-LAMP assays using RNA standards. Two replicates were performed for each primer set. The replicates of the same primer set often generated completely overlapped amplification curves. A short bar was used to highlight the non-overlapping curves of the same primer. The curves of non-template control (NTC) are not shown. *The Tt values of the Set-8 were obtained by another repeated comparative experiments with Set-5and Set-9, both of which showed a consistent trend, but slightly lower Tt values than those shown here.