Figure 3
Comparison of performance of 10 selected RT-LAMP primer sets using 41 clinical RNA samples. (A) Positive rates and Tt values of 10 selected RT-LAMP assays. The 41 clinical samples included 29 SARS-CoV-2 positive and 12 negative clinical samples that were previously determined by RT-qPCR assay28. The positive rate was calculated by dividing the number of positive sample by each primer set by total positive sample number of RT-qPCR assay (i.e. 29). (B) Paired comparison of Tt values of the primers Set-4, Set-10, Set-11, Set-13, Set-14 and Set-17. Because all RNA from clinical samples were fourfold diluted and some of them have very low viral load (high Ct values by RT-qPCR assay), some positive samples were not detected as positive by the RT-LAMP assay, which are defined as false-negative. We calculated the concordance rate by dividing the number of consistent results (true positive, true negative and false-negative) by any two primer sets by the total sample number (i.e. 41). SD standard deviation.
