Table 2 Primers used in the study.
Primer targeted region | Primer sequence | Purpose | |
|---|---|---|---|
IgH major | cacgacgttgtaaaacgacCAGCCTGAGAGCCGAGGACAC | PARR | |
TGAGGAGACGGTGACCAGGGT | |||
IgH minor | cacgacgttgtaaaacgacCAGCCTGAGAGCCGAGGACAC | PARR | |
TGAGGACACAAAGAGTGAGG | |||
TCR | cacgacgttgtaaaacgacCGTGTACTACTGCGCTGCCTGG | PARR | |
TGTGCCAGGACCAAGCACTTTGTT |
Primer targeted region | MIQE context | Purpose | Elongation temperature (°C) |
|---|---|---|---|
PTPN11 hot spot 1 (E76K) | GATTCAGAACACTGGTGATTACTATGACTTGTATGGAGGGGAAAAGTTTGCCACGTTGGCT[G/A]AGTTGGTCCAGTATTATATGGAACATCACGGACAATTAAAAGAGAAGAATGGAGATGTTAT | ddPCR: mutation detection of PTPN11 E76K | 58 |
PTPN11 hot spot 2 (G503V) | GGTGTTGACTGCGACATTGATGTTCCCAAAACCATTCAGATGGTGCGGTCTCAGAGGTCAG[G/T]GATGGTCCAGACAGAAGCACAGTACCGATTCATTTATATGGCTGTCCAGCATTACATTGAA | ddPCR: mutation detection of PTPN11 G503V | 58 |
TRPM7 | CATTACCTGTGTTTACTCCTCCAGTTAAAATCCAGGCTCCGGTTGTAACTGCAGCTTTAATAAGACCCTTTCCAAGCAGCTGCTTGATTCGTGGGTGAAGCTCAAATTTCTGCATGCCTCCAT | ddPCR: Copy Number determination | 58 |
MdM2 | TGATCTTCTAGGAGATTTGTTTGGAGTGCCAAGCTTCTCTGTGAAAGAGCACAGGTAAATTCTTCAGTTTAGTCCGTTGTAAAAAGCCAGCTGGGCAAACATTTCAGTTTACCTCCTCCTTTT | ddPCR: Copy Number determination | 58 |
chr9 control | GCAGTCACCCCATCATCGTCCCGGCAAGGAAGCTTGTGCACAAACGCCAGCATCTGGTACTTGGCCCTGATGAATTCTGACTTGATGGGGCTGGGCAGGGCGCATAGGATGCGACGGTGAGCA | ddPCR: Copy Number determination | 58 |
Specific probe for dog 1 | F: CGGCtgTcTATTACTGTGCg | ddPCR MRD follow-up | 60 |
R: CTGCACCAGGACAAACAGAA | |||
56-FAM/TGTCACCCA/ZEN/ATTGGAGCTGATCCC | |||
Specific probe for dog 2 | F: ACTACCTCCGTGCTTTTGGT | ddPCR MRD follow-up | 60 |
R: CGTGTCCTCGGCTCTCAG | |||
56-FAM/CGGCCGTAT/ZEN/ATTATTGTGTGAAGGC | |||
Specific probe for dog 3 | F: ACACGGCCGTCTATTACTGT | ddPCR MRD follow-up | 60 |
R: GTTCCTGGCCCCAATAGTCA | |||
56-FAM/ACGGCCGTC/ZEN/TATTACTGTTTCGGG | |||
Specific probe for dog 4 | F: CGACGGTAGCTACGGTAACAAT | ddPCR MRD follow-up | 60 |
R: GGTCTCACCCGCACAGTAAT | |||
56-FAM/AGACCGACG/ZEN/GTAGCTACGGTAACA | |||
chr26 control | F: TGAGACAACCCTCCC | ddPCR MRD follow-up | 60 |
R: CTTCTCTCGCTGAGAAAG | |||
5HEX/CTGCCCGCT/ZEN/CTTTGATCCTGAGTC |
