Figure 6

PC-controllable system for feedback control of LDs of adipose cellular tissue. (a) A total system composition consisting of subsystems for culture, biochemical stimulation, and imaging. Size change of LDs of adipose cellular tissue was monitored when biochemical stimulation using accelerator and decelerator. (b) Flow chart of an algorism for feedback control of LD size by controlling biochemical stimulation. (c) Photographs of continual images of adipose tissue and LDs according to time. Typical adipose tissue with LDs was indicated by using a red circle in (c). Cluster of LDs distributed and changed its shape and location in a frame. (d) The relative change in the LDs content obtained through the image processing by binarizing and thinning according to time (n = 6). The biochemical stimulation was automatically switched when the ratio of LDs changed over a set value as a threshold. The threshold was set at 0.3% of change in the ratio in the experiment. The feedback control of the size of LDs was successfully performed. The biochemical decelerator was automatically supplied when the ratio of LDs increased over a set value as a threshold, whereas accelerator was automatically supplied when LDs decreased over the threshold.