Figure 2
From: Sulf2a controls Shh-dependent neural fate specification in the developing spinal cord

V3 interneuron production is increased upon Sulf2a depletion. Side views of 48 hpf embryos. (a–f) Detection (a–e) and quantification (f) of sim1a-expressing neurons in embryos injected with ctrlMO (a, n = 34), sulf2aMOATG (b, n = 20) or sulf2aMOsplice (c, n = 34) and in wild-type (d, n = 10) or sulf2a−/− (e, n = 12) embryos from two independent experiments in each case. Datasets were compared with Mann Whitney’s test (two-tailed). Data are presented as mean number of cells per embryo ± s.d (**p < 0.01, ****p < 0.0001).