Figure 2
From: No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor
Basigin expressed as recombinant protein ectodomains retain their biochemical activity. (a) Expression and purification of two and three Ig-like domain isoforms of basigin. Proteins were resolved under reducing conditions by SDS-PAGE and stained with a Coomassie dye (b). Recombinant basigin but not control proteins are recognized by anti-basigin monoclonal antibodies. ELISA dilution series of BSG and BSG-long recognized by three different monoclonal antibodies, and a control OX68 antibody against their tags. A negative control of a recombinant Cd4 tag is included for each antibody. (c) Recombinant basigin retains folded conformation of epitopes recognized by three different monoclonal antibodies. ELISA dilution curves comparing unmodified basigin to protein treated with heat and a reducing agent. Three replicates were performed for all ELISA curves except MEM-M6/6, for which only a single trial was done. Dose response curve model fit lines are superimposed on the data points, with shading indicating the 95% confidence bounds of the models.
