Table 2 Limit of quantification for engineered sample mixtures of A. racemosa using qPCR and dPCR.
Actual mix ratio of A. racemosa (%) | qPCR | dPCR | |||||||
|---|---|---|---|---|---|---|---|---|---|
LC-480 | bCUBE | ||||||||
Measured ratio (%) | RSD (%) | Bias (%) | Measured ratio (%) | RSD (%) | Bias (%) | Measured ratio (%) | RSD (%) | Bias (%) | |
100% | 100 | 0.20 | 0.00 | 100 | 0.48 | 0.00 | 100 | 0.67 | 0.00 |
90% | 98 | 0.45 | 8.90 | 93 | 0.64 | 3.52 | 88 | 0.88 | − 2.20 |
80% | 74 | 0.36 | − 7.90 | 77 | 0.16 | − 3.75 | 74 | 0.44 | − 11.70 |
50% | 54 | 0.27 | 8.00 | 53 | 0.19 | 6.33 | 36 | 1.30 | − 28.00 |
10% | 11 | 1.47 | 10.00 | 12 | 0.50 | 15.00 | 11 | 3.30 | 10.00 |
