Table 1 Characteristics of the paired-end raw sequences obtained after Illumina MiSeq sequencing of As Burgas water before and after quality control (QC) with PRINSEQ.
BW1 | BW2 | ||||
|---|---|---|---|---|---|
Read 1 | Read 2 | Read 1 | Read 2 | ||
Before PRINSEQ QC | |||||
Number sequences | 867,096 | 867,096 | 873,846 | 873,846 | |
Total bases | 227,341,174 | 232,496,706 | 230,953,710 | 235,685,441 | |
Seq. length (bp) | 262.19 ± 46.53 | 268.13 ± 47.31 | 264.30 ± 44.08 | 269.71 ± 45.04 | |
Mean GC content (%) | 54.95 ± 11.23 | 55.32 ± 11.61 | 54.09 ± 11.76 | 54.46 ± 12.20 | |
Number of pairs | 867 096 (100% sequences) | 873 846 (100% sequences) | |||
After PRINSEQ QC | |||||
Number sequences | 747,684 | 747,684 | 761,635 | 761,635 | |
Total bases | 193,410,210 | 192,903,192 | 199,007,260 | 198,412,539 | |
Seq. length (bp) | 258.68 ± 46.62 | 258.00 ± 45.55 | 261.29 ± 43.84 | 260.51 ± 42.86 | |
Mean GC content (%) | 54.22 ± 11.41 | 54.51 ± 11.63 | 53.31 ± 11.87 | 53.60 ± 12.11 | |
Number of pairs | 747,684 (100% sequences) | 761,635 (100.00% sequences) | |||
