Table 3 Analysis of Subsystems at level 3. From the 28 subsystems at level 3 registered by MG-RAST, only those subsystems with more than 2000 reads assigned were collected in the table.

From: Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases

SEED subsystems

No. of reads

Level 1

Level 2

Level 3

BW1

BW2

Amino acids and derivatives

Branched-chain amino acids

branched-chain_amino_acid_biosynthesis

2799

2930

Lysine, threonine, methionine, and cysteine

methionine_biosynthesis

3119

3134

Carbohydrates

CO2 fixation

Calvin-benson_cycle

2204

2351

One-carbon Metabolism

Serine-glyoxylate_cycle

3311

3219

Cell Wall and capsule

NULL

Peptidoglycan_biosynthesis

2039

2106

Clustering-based subsystems

NULL

Bacterial_cell_division

2293

2119

Cofactors, vitamins, prosthetic groups, pigments

Tetrapyrroles

Heme_and_siroheme_biosynthesis

2244

2205

Folate and pterines

YgfZ

2527

2741

DNA metabolism

DNA replication

DNA-replication

2446

2332

DNA repair

DNA_repair,_UvrABC_system

2038

2019

DNA repair

DNA_repair,_bacterial

2532

2661

Fatty acids, lipids, and isoprenoids

Fatty acids

Fatty_Acid_biosynthesis_FASII

2365

2459

Membrane transport

ABC transporters

ABC_transporter_branched-chain_amino_acid_(TC_3.A.1.4.1)

2598

2380

Motility and chemotaxis

Flagellar motility in prokaryota

Flagellum

3061

2874

Nitrogen metabolism

NULL

Ammonia_assimilation

2028

2279

NULL

Nitrate_and_nitrite_ammonification

4965

4169

Nucleosides and nucleotides

Purines

De_novo_purine_biosynthesis

2868

3365

Purines

Purine_conversions

2695

2672

Phosphorus metabolism

NULL

Phosphate_metabolism

3204

3396

Protein metabolism

Protein folding

Protein_chaperones

2584

2551

Protein degradation

Proteolysis_in_bacteria,_ATP-dependent

2054

1933

Protein biosynthesis

Ribosome_LSU_bacterial

4896

4258

Protein biosynthesis

Ribosome_SSU_bacterial

3084

2958

Protein biosynthesis

Universal_GTPases

2110

2012

Respiration

Electron donating reactions

Respiratory_complex_I

5524

5578

Electron accepting reactions

Terminal_cytochrome_C_oxidases

3054

2826

RNA metabolism

Transcription

RNA_polymerase_bacterial

3475

3402

RNA processing and modification

tRNA_modification_archaea

1801

2035

Sulfur metabolism

NULL

Sulfur_oxidation

3112

2665