Table 4 Primers used for the amplification of T. scotoductus β-galactosidase and putative β-galactosidase ORFs.
ORF amplified | Name | Sequence |
|---|---|---|
T.scotoductus β-gal | MECA01f | 5′ GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGAAGCTGGACCCCAACCATCCC 3′ |
MECA02r | 5′ GGGGACCACTTTGTACAAGAAAGCTGGGTCCTACTCCCAAAGCACCCGCCT 3′ | |
T.scotoductus putative β-gal | MECA03f | 5′ GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGAGGTGGGAAAGAGCTTGGTTTTTGG 3′ |
MECA04r | 5′ GGGGACCACTTTGTACAAGAAAGCTGGGTCTCACCAGGCCACCCCCAGG 3′ |
