Figure 4 | Scientific Reports

Figure 4

From: Stimulatory, but not anxiogenic, doses of caffeine act centrally to activate interscapular brown adipose tissue thermogenesis in anesthetized male rats

Figure 4

Photomicrographs illustrating the rostrocaudal levels of the paraventricular hypothalamic nucleus anterior parvo (PaAP), medial preoptic area (MPA), lateral hypothalamus (LH) delineation of regions by immunostaining of cFos ir (dark speckled puncta on image): (A) − 1.30 mm bregma of caffeine treated rats; (B) − 1.30 mm bregma of vehicle treated rats; (C) represent -1.40 mm of caffeine treated rats; (D) − 1.40 mm bregma of vehicle treated rats. Dashed lines represent regions of interest. 3 V 3rd ventricle. Scale bar, 200 µm.

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