Figure 1 | Scientific Reports

Figure 1

From: Lactobacillus rhamnosus attenuates bone loss and maintains bone health by skewing Treg-Th17 cell balance in Ovx mice

Figure 1

LR-CM inhibits osteoclastogenesis in a dose dependent manner: Osteoclasts differentiation was induced in Bone Marrow Macrophages (BMMs) with M-CSF (30 ng/ml) and RANKL (100 ng/ml) with or without Lactobacillus rhamnosus—conditioned media (LR-CM) at different ratios of 1:10 and 1:1 for 6 days. Giant multinucleated cells were stained with TRAP and cells with ≥ 3 nuclei were considered as mature osteoclasts. (A) Photomicrographs at different magnifications (10×, 20× and 40×) were taken. (B) Number of TRAP positive cells. (C) Number of TRAP positive cells with more than 3 nuclei. (D) Area of osteoclasts. The above images are indicative of one independent experiment and similar results were obtained in three different independent experiments. Statistical significance was considered as p ≤ 0.05 (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) with respect to indicated groups.

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