Figure 9

LR modulates Treg and Th17 cell differentiation in vitro. (A) For Tregs differentiation, splenic naïve CD4⁺ T cells stimulated with anti-CD3 and anti-CD28 mAbs were incubated with TGF-β1 and IL-2 with or without LR-CM. After 4 days, cells were analysed for Foxp3 expression by FACS. (B) Average percentage of CD4⁺Foxp3⁺ cells from two independent experiments of A. (C) MFI showing the expression of Foxp3 in CD4⁺ T cells. (D) For Th17 differentiation, splenic naïve CD4⁺ T cells stimulated with anti-CD3 and anti-CD28 mAbs, incubated with TGF-β1, IL-6, IL-23 with or without LR-CM. (E) Average percentage of CD4⁺Roryt⁺ cells from three independent experiments of D. (F) MFI showing the expression of Roryt in CD4⁺ T cells. Data is reported as Mean ± SEM. Similar results were obtained in two independent experiments. MFI, mean fluorescence intensity. Statistical significance of each parameter was assessed by ANOVA followed by paired group comparison. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with indicated groups.