Figure 7
Binding of TP4-A12I,A15I peptides and drug to protein D. (a) Emission spectra of proteins A and D in the presence of sarkosyl, TP4-A12I,A15I, Ibuprofen and Phenytoin. Reagents were added to the protein A and protein D at the indicated concentrations and excited at 280 nm and recorded for the emission spectrum between 300 to 450 nm. Protein A and D: 0.75 μM each; Sarkosyl: lines 1–5 at 0, 5, 20, 80 and 320 μM; TP4-A12I, A15I: lines 1 to 4 at 0, 30, 60, 120 μM; Ibuprofen; lines 1 to 5 at 0, 3, 6, 12 and 24 μM; Phenytoin; lines 1 to 5 at 0, 2.5, 5, 10 and 20 μM. (b) Inhibition of the binding of TP4-A12I,A15I to protein D by Ibuprofen. The protein A and D mixture (20 μg each) was pre-incubated with Ibuprofen, Warfarin or Phenytoin for 30 min before incubating with TP4-A12I,A15I (4 μg) at 37 °C for 30 min, and analyzed by native PAGE, pH8.8.
