Figure 4
From: Further evidence supporting a potential role for ADH1B in obesity
ADH1B protein expression decreased with increasing BMI. (A) Representative composite immunoblot image of cell lysates from 3 pre-adipocyte cell lines derived from lean (BMI < 30 kg m−2) or obese (BMI ≥ 30 kg m−2) individuals during adipogenesis. Total protein was isolated at the indicated time points during differentiation, resolved by SDS-PAGE, and immunoblotted with antibodies specific for ADH1B and β-Actin loading control. Representative full-length blot is presented in Supplementary Figure S14. (B) Quantitative analysis of ADH1B expression. Western blot analysis was performed at least four times. Fluorescence intensity was quantified and normalized to β-Actin control. Data is presented as the mean ± s.e.m. AU, Arbitrary Units.
