Table 4 Four different GBA1 primer sets that will lead to amplification of the functional gene and not the pseudogene GBAP1.
From: False negatives in GBA1 sequencing due to polymerase dependent allelic imbalance
GBA1 primer set | Name | Sequence (5′- > 3′) | Start genomic position |
|---|---|---|---|
1 Mata et al.20 | GBA_P1_F | GTTGTCACCCATACATGCCC | chr1: 155204150 |
GBA_P1_R | CTCTCATGCATTCCAGAGGC | chr1: 155211199 | |
Product length | 7050 | – | |
2 Jeong et al.24 | GBA_P2_F | TCCTAAAGTTGTCACCCATACATG | chr1: 155202292 |
GBA_P2_R | CCAACCTTTCTTCCTTCTTCTCAA | chr1: 155211206 | |
Product length | 8915 | – | |
3 Liu et al.18 | GBA_P3_F | CGACTTTACAAACCTCCCTG | chr1: 155203972 |
GBA_P3_R | CCAGATCCTATCTGTGCTGG | chr1: 155211737 | |
Product length | 7766 | – | |
4 Designed by GenomeScan using NCBI primer blast | GBA_P4_F | AGAAGTTGATCCCGGTGCTG | chr1: 155202617 |
GBA_P4_R | ATGATGAAACAAGGGACGCTGC | chr1: 155211676 | |
Product length | 9060 | – |
