Figure 3

Experimental protocol for exposure of fish to ethanol. The experimental environment was configured based on the literature4. (a) Measurement aquarium, divided into three sections using gauze with different colour patterns. (b) Flow chart of the place preference conditioning protocol. The initial place preference is measured on the first day. The fish is conditioned on the second day to induce a place preference. The ventilatory signals and motion on the first and third day were analysed and compared.