Figure 7

Starving foxO (RNAi) animals show tissue disruption and a reduction of cell death. (a) Schematic depicting RNAi procedure. (b) 50% of in vivo foxO knockdown animals presented unpigmented regions (white arrow), neural tissue disappearance (yellow arrow in synapsin immunoassayed animals, and pharynx size reduction, as shown by DAPI staining and relative size quantification (controls, n = 17; RNAi, n = 18; *P < 0.05). (c) Quantification of TUNEL + cells on transversal sections show a reduction of positive cells in foxO (RNAi) animals compared to controls (controls, n = 86; RNAi, n = 117; *P < 0.05). (d) Quantification of caspase-3 activity in foxO (RNAi) animals and controls shows cell death reduction (controls, n > 6; RNAi, n > 6; **P < 0.01). Scale bars: b up and mid = 100 µm and down = 10 µm; c = 100 µm.