Figure 5

Reaction template-concentration optimization. Template-concentration optimization (10 ng to 50 fg) result was visualized using EtBr and basic fuchsin (a). Further, the result was confirmed on a 2% agarose gel (b), where the lanes (1 to 7) indicate template DNA concentrations (10 ng, 1 ng, 100 pg, 50 pg, 10 pg, 100 fg, and 50 fg), lane N indicates negative control, and the ane L indicates 1 kb ladder (Thermo Scientific).