Figure 2

Fine mapping of qGL11. (a) Graphic genotype of N232 showing a single substitution segment encompassing qGL11. (b) Comparison of the grain size in ZS97 and N232. Differences between ZS97 and N232 in grain length, grain width, grain thickness and thousand-grain weight. Data are given as the mean and SE (n = 6 individuals). (c) The QTL was detected in a CSSL-derived F₂ population. PVE, Add and Dom respectively represent phenotypic variation explained, additive effect and dominant effect. (d) Fine mapping of qGL11 in a 25-kb region using a large segregating population (n = 3500). Five predicted genes are indicated, including Os11g0528700 (700) which was selected as the candidate gene. L3-L10 represent recombinant plants. Phenotype data for GL (grain length) and TGW (thousand-grain weight) are provided as the mean and SE (n = 10). (e) Quantitative RT-PCR analysis of gene expression in the 8-cm panicle. Transcription levels relative to NIL-ZS97, which was set to 1, are presented as the mean and SE of triplicates. LOC_Os03g13170 (Ubiquitin) is the control gene. (f) Sequence comparison of Os11g0528700 among four cultivars. The triangle indicates a nucleotide deletion. **, * and NS indicate significant differences at P < 0.01, P < 0.05, and no significance, respectively, by Student’s t-test.