Figure 5 | Scientific Reports

Figure 5

From: Identification of a novel QTL and candidate gene associated with grain size using chromosome segment substitution lines in rice

Figure 5

CRISPR/Cas9-induced mutants of the gene and their phenotypes. (a) Schematic gene model of OsGH3.13 showing the sgRNA target site and sequence. The protospacer-adjacent motif (CGG) is shown in red font. (b, c) Homozygous mutations of OsGH3.13 in 9311 (b), and ZH11 (c). Scale bars: 10 mm. (d–m) Differences between the mutants and corresponding wild type in grain size and grain yield per plant. Data are given as the mean and SE (n = 6). Asterisks (**) indicate significant difference from the wild type by Student’s t-test at P < 0.01. NS, no significance.

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