Figure 4
The effect of Mlig-TIM29(RNAi) on the number of mitotic cells and gene expression. (a) Mlig-TIM29(RNAi) causes a significant decrease in the number of mitotic cells in regenerating worms (Regeneration), but not in uncut worms (Homeostasis whole body). To allow comparison between cut and uncut worms, the number of mitotic cells was quantified in the head and pharynx region of uncut worms (Homeostasis anterior of testes) which corresponds with the fragment left after amputating the body. In control gfp(RNAi) worms, regeneration causes a significant increase in the number of mitotic cells. This is not observed in Mlig-TIM29(RNAi) worms. n.s.: p > 0.05, ***p < 0.001 (ANOVA and post-hoc Tukey test). (b) Genes differentially expressed between gfp(RNAi) and Mlig-TIM29(RNAi) worms during homeostasis (uncut worms) and regeneration (cut worms).
