Figure 3
AITRL is a transcription repressor. (a) Subcellular localization of AITRL. Plasmids of the GFP-AITRL effector gene and the NLS-RFP nuclear marker gene were co-transfected into Arabidopsis protoplasts isolated from leaves of the Col wild type. The transfected protoplasts were incubated in darkness at room temperature for 16–18 h. The GFP and RFP fluorescence was observed under a fluorescence microscope. Co-transfection of GFP effector gene and the NLS-RFP nuclear marker gene was use as a control. (b) Transcriptional activities of AITRL. Plasmids of the LexA-Gal4:GUS reporter gene, the LD-VP transcription activator gene and the GD-AITRL effector gene were co-transfected into Arabidopsis protoplasts isolated from leaves of the Col wild type plants. Co-transfection of the GD effector gene was used as a control. The transfected protoplasts were incubated in darkness at room temperature for 20–22 h. GUS activities were measured by using a microplate reader. Data represent the mean ± SD of three replicates. *Significantly different from the GD (p < 0.005).
