Figure 6
Expression of ABA signaling component genes in the Col wild type, the 35S:AITRL transgenic plants and the aitrl mutants. Expression of PP2C genes (a), PYL genes (b) and SnRK2 genes (c) in response to ABA in the Col wild type, the 35S:AITRL transgenic plants and the aitrl mutants. Twelve-day-old seedlings were treated with 50 µM ABA or solvent methanol as a control for 4 h. Total RNA was isolated and qRT-PCR was used to examine the expression of the ABA signaling component genes. The expression of ACT2 was used as an inner control. Fold changes were calculated by comparing the expression levels of the corresponding genes in ABA treated and the control seedlings. Data represent the mean ± SD of three replicates. *Significantly different from the wild type (p < 0.05).
