Figure 8

AG1529 reduces algesically-sensitized capsaicin-induced nociceptor excitability. (A) Representative MEA recordings of capsaicin-evoked APs and its sensitization by instillation of 50 µM histamine 2 min before the third capsaicin pulse, along with the effect of 10 µM capsazepine or 1 µM AG1529 added 2 min before the third capsaicin pulse (P3). (B) Similar protocol that in (A) but sensitization was triggered by an inflammatory soup containing 50 µM histamine, 1 µM bradykinin and 10 µM ATP. (C,D) Normalized capsaicin-induced firing of the third pulse (P3) with respect to the first one (P1) for the different treatments. Data are expressed as mean ± SEM, and analysed using the One-Way Anova (F(3,553) = 28.28, p < 0.0001 for panel (C); and F(3,794) = 144.5, p < 0.0001 for panel D) followed by the Bonferroni´s post-hoc test, p-values for statistical difference are indicated. N (independent experiments) = 3, nº of electrodes = 50–200). Data of vehicle without histamine or inflammatory soup were obtained in independent experiment, N = 3 and number of electrodes 120.