Figure 6
From: Gene expression and functional abnormalities in XX/Sry Leydig cells
Steroidogenic gene expression is affected in XX/Sry ALCs. (a) Expression of steroidogenic genes as well as Ad4BP/SF-1 and Lhcgr in the XY and XX/Sry ALC transcriptome datasets. CPMs are the means of biological triplicates. Increased and decreased gene expression in the XX/Sry ALCs is indicated in red and blue, respectively, with deeper shading for larger differences. (b) Expression of the genes shown in (a) was validated by qRT-PCR. The data were normalized to Actb and are presented as means ± SEM. Three biologically independent samples (n = 3) were used. * p < 0.05, ** p < 0.01, *** p < 0.001, using Student’s t-test (b). R software version 3.4.3 (https://www.r-project.org) was used to draw the plots in (b).
