Figure 7
From: Gene expression and functional abnormalities in XX/Sry Leydig cells
17,20-lyase activity of CYP17A1 is reduced in XX/Sry testes. (a) The pathway of testosterone synthesis from cholesterol. The enzymes implicated in the pathway are shown in gray boxes. P5, pregnenolone; P4, progesterone; 17αOH-P5, 17α-hydroxy-pregnenolone; 17αOH-P4, 17α-hydroxyprogesterone; DHEA, dehydroepiandrosterone; A-dione, androstenedione; A-diol, androstenediol; T, testosterone. (b) The quantities of these steroids in the XY and XX/Sry testes were determined using GC–MS/MS. Eight biologically independent samples (n = 8) were used in this analysis. (c) Metabolic ratios for all reactions were calculated as the ratio of substrate to metabolite using the quantities of intratesticular steroids detected in the XY and XX/Sry testes. (d) Expression of Por and Cyb5a genes were examined using qRT-PCR. The data were normalized to Actb and are presented as means ± SEM. Three biologically independent samples (n = 3) were used for each cell type. * p < 0.05, ** p < 0.01, *** p < 0.001, using Mann–Whitney U test (b,c) and Student’s t-test (d). R software version 3.4.3 (https://www.r-project.org) was used to draw the plots in (b–d).
