Figure 12

Effects of SFN on miR-146a, miR-155 and miR-21 miRNAs expression levels in LPS/IFN- γ-stimulated RAW 264.7 macrophages. Cells were treated for 6 h with SFN (5 and 20 µM) in the presence of LPS (10 ng/mL) plus IFN-γ (10 U/mL) with DOX (0.1 µM. The expression levels of miR-146a (a), miR-155 (b), and miR-21 (c) were measured using qPCR and were normalized to RNU6. Data are expressed as mean ± S.E. (n = 3). Comparisons are made with ANOVA followed by Student–Newman–Keuls (SNK) post-hoc test; *, P < 0.05, compared with control; $, P < 0.05, compared with LPS/IFN-γ plus DOX treatment.