Figure 4

Effects of the activemonomers of SWTX on H₂O₂-induced H9c2 cell oxidative injury. (A) H9c2 cells treated with 200–1800 μΜ H₂O₂ for 22 h, shown marked reductions with increasing dose in cell viability (n = 6). (B) 12.5, 50, 200 μΜ G3, G8, R3, R5 and T1 pretreated H9c2 cell for 2 h and co-incubated with 900 μΜ H₂O₂ for additional 22 h, significantly improved H9c2 cell viability in comparison to the cells singly treated with 900 μΜ H₂O₂, among them, G8 and T1 shown best (n = 6). (C) The protective ability of 50μΜ G8, R3, R5 and T1 groups is significantly reduced by PI3K inhibitor LY294002 (50 nM) in H9c2 cell (n = 6). (D) The cell apoptosis ratio was detected by flow cytometry, and the data indicated a decreasing percentage of cells in the lower and upper right quadrants (early and late stage apoptosis) in 50 μM G3, G8, R2, R7 and T1 groups compared with 900 μΜ H₂O₂ group, and an increasing percentage of the apoptosis cells following LY294002 (50 nM) treatment . ^##P < 0.01 versus control group; **P < 0.01 versus model; *P < 0.05 versus model; ^&&P < 0.01 monomer versus LY294002 + monomer and ^&P < 0.05 monomer versus LY294002 + monomer.