Table 1 Isolation of Lactobacillus reuteri strains, and determination of 1,3-PDO and reuterin production. 1,3-PDO and reuterin production were determined in Lactobacillus reuteri isolates using HPLC and Colorimetric method, respectively.

From: Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria

No	ID	Strain	Origin	Residual glucose (g/L)	Residual glycerol (g/L)	1,3-PDO production (g/L)^a	Reuterin production^b
1	PB3	Lactobacillus reuteri	Swine feces	1.96	6.93	++	+
2	PB6	Lactobacillus reuteri	Swine feces	0.16	5.51	+++	+
3	PF4	Lactobacillus reuteri	Swine feces	1.18	4.96	+++	–
4	PPF3	Lactobacillus reuteri	Swine feces	2.76	8.10	++	–
5	PMA2	Lactobacillus reuteri	Swine feces	0.05	21.49	–	–
6	PMF1	Lactobacillus reuteri	Swine feces	0.64	20.99	–	–
7	PMF2	Lactobacillus reuteri	Swine feces	0.57	21.45	–	–
8	AN306	Lactobacillus reuteri	Small intestine of swine	0.35	21.47	–	–
9	AN313	Lactobacillus reuteri	Small intestine of swine	0.56	20.84	–	–
10	AN403	Lactobacillus reuteri	Small intestine of swine	4.51	21.47	–	–
11	AN413	Lactobacillus reuteri	Small intestine of swine	0.05	6.05	–	–
12	AN417	Lactobacillus reuteri	Small intestine of swine	1.08	20.40	–	–
13	AN507	Lactobacillus reuteri	Small intestine of swine	0.45	20.09	–	–
14	AN509	Lactobacillus reuteri	Small intestine of swine	0.08	21.43	–	–
15	AN510	Lactobacillus reuteri	Small intestine of swine	2.21	21.50	–	–
16	AN511	Lactobacillus reuteri	Small intestine of swine	0.28	21.42	–	–
17	AN513	Lactobacillus reuteri	Small intestine of swine	5.19	21.26	–	–
18	AN516	Lactobacillus reuteri	Small intestine of swine	1.39	21.48	–	–
19	AN519	Lactobacillus reuteri	Small intestine of swine	0.20	21.30	–	–
20	AN521	Lactobacillus reuteri	Small intestine of swine	0.18	21.17	–	–
21	AN523	Lactobacillus reuteri	Small intestine of swine	1.23	21.30	–	–
22	AN525	Lactobacillus reuteri	Small intestine of swine	0.13	21.28	–	–
23	AN527	Lactobacillus reuteri	Small intestine of swine	0.12	20.95	–	–
24	AN530	Lactobacillus reuteri	Small intestine of swine	0.46	21.21	–	–
25	AN540	Lactobacillus reuteri	Small intestine of swine	1.00	21.36	–	–
26	AN543	Lactobacillus reuteri	Small intestine of swine	0.10	21.42	–	–
27	AN546	Lactobacillus reuteri	Small intestine of swine	3.58	21.42	–	–
28	AN548	Lactobacillus reuteri	Small intestine of swine	0.24	21.32	–	–
29	AN703	Lactobacillus reuteri	Small intestine of swine	8.13	21.46	–	–
30	AN704	Lactobacillus reuteri	Small intestine of swine	0.42	21.37	–	–
31	AN705	Lactobacillus reuteri	Small intestine of swine	1.38	20.89	–	–
32	AN708	Lactobacillus reuteri	Small intestine of swine	5.99	21.38	–	–
33	AN709	Lactobacillus reuteri	Small intestine of swine	3.36	21.42	–	–
34	AN711	Lactobacillus reuteri	Small intestine of swine	2.64	21.41	–	–
35	AN722	Lactobacillus reuteri	Small intestine of swine	4.81	21.24	–	–
36	AN724	Lactobacillus reuteri	Small intestine of swine	0.12	21.34	–	–
37	AN727	Lactobacillus reuteri	Small intestine of swine	2.48	21.22	–	–
38	AN832	Lactobacillus reuteri	Small intestine of swine	7.69	21.39	–	–
39	AN903	Lactobacillus reuteri	Small intestine of swine	1.03	21.47	–	–
40	AN904	Lactobacillus reuteri	Small intestine of swine	3.63	21.49	–	–
41	AN906	Lactobacillus reuteri	Small intestine of swine	9.73	18.93	–	–
42	AN919	Lactobacillus reuteri	Small intestine of swine	12.51	18.90	–	–
43	AN924	Lactobacillus reuteri	Small intestine of swine	7.59	18.78	–	–
44	AN925	Lactobacillus reuteri	Small intestine of swine	9.31	19.01	–	–
45	AN926	Lactobacillus reuteri	Small intestine of swine	9.90	21.05	–	–
46	AN933	Lactobacillus reuteri	Small intestine of swine	4.88	19.54	–	–
47	AN941	Lactobacillus reuteri	Small intestine of swine	10.13	19.27	–	–
48	AN943	Lactobacillus reuteri	Small intestine of swine	0.75	20.79	–	–
49	AN947	Lactobacillus reuteri	Small intestine of swine	9.92	18.72	–	+
50	AN1002	Lactobacillus reuteri	Small intestine of swine	12.09	19.00	–	–
51	AN1009	Lactobacillus reuteri	Small intestine of swine	9.69	18.99	–	–
52	AN1016	Lactobacillus reuteri	Small intestine of swine	4.47	20.87	–	–
53	AN1019	Lactobacillus reuteri	Small intestine of swine	8.83	20.52	–	–
54	AN1026	Lactobacillus reuteri	Small intestine of swine	7.55	19.04	–	–
55	AN1033	Lactobacillus reuteri	Small intestine of swine	2.38	20.96	–	–
56	AN1034	Lactobacillus reuteri	Small intestine of swine	5.58	19.42	–	–
57	AN1109	Lactobacillus reuteri	Small intestine of swine	8.60	21.03	–	–
58	SBF301	Lactobacillus reuteri	Infant feces	0.51	5.70	++	+
59	SBF302	Lactobacillus reuteri	Infant feces	1.30	7.37	++	+
60	SBF303	Lactobacillus reuteri	Infant feces	1.04	7.09	++	++
61	SBF304	Lactobacillus reuteri	Infant feces	2.31	8.33	++	+
62	SBF305	Lactobacillus reuteri	Infant feces	10.85	15.80	+	+
63	SBF306	Lactobacillus reuteri	Infant feces	0.32	5.98	+++	+
64	SBF307	Lactobacillus reuteri	Infant feces	0.59	6.27	+++	++
65	SBF309	Lactobacillus reuteri	Infant feces	3.99	9.26	++	–
66	SBF310	Lactobacillus reuteri	Infant feces	3.57	9.09	++	+
67	SBF311	Lactobacillus reuteri	Infant feces	0.08	5.20	++	++
68	SBF312	Lactobacillus reuteri	Infant feces	4.47	10.15	++	+
69	SBF313	Lactobacillus reuteri	Infant feces	7.43	12.87	++	+
70	SBF314	Lactobacillus reuteri	Infant feces	5.78	11.30	++	+
71	SBF315	Lactobacillus reuteri	Infant feces	4.40	9.93	++	+
72	SBF316	Lactobacillus reuteri	Infant feces	1.33	8.92	++	–
73	SBF317	Lactobacillus reuteri	Infant feces	12.47	16.74	+	–
74	SBF318	Lactobacillus reuteri	Infant feces	5.78	11.44	++	+
75	SBF320	Lactobacillus reuteri	Infant feces	11.91	16.46	+	+
76	SBF321	Lactobacillus reuteri	Infant feces	9.37	14.92	+	+
77	SBF325	Lactobacillus reuteri	Infant feces	0.32	5.27	+++	+
78	SBF326	Lactobacillus reuteri	Infant feces	6.18	11.47	++	+
79	SBF327	Lactobacillus reuteri	Infant feces	5.54	10.86	++	+++
80	SBF328	Lactobacillus reuteri	Infant feces	0.95	14.94	+	+++
81	SBF329	Lactobacillus reuteri	Infant feces	1.13	7.73	+++	–
82	SBF330	Lactobacillus reuteri	Infant feces	8.63	14.70	+	+
83	SBF331	Lactobacillus reuteri	Infant feces	0.39	6.30	+++	++
84	SBF332	Lactobacillus reuteri	Infant feces	0.77	7.17	+++	++
85	SBF333	Lactobacillus reuteri	Infant feces	1.59	8.07	+++	+++
86	SBF334	Lactobacillus reuteri	Infant feces	1.65	8.12	++	++
87	SBF335	Lactobacillus reuteri	Infant feces	0.32	6.50	+++	+++
88	SBF336	Lactobacillus reuteri	Infant feces	4.58	10.57	++	++
89	SBF401	Lactobacillus reuteri	Infant feces	0.29	6.41	+++	+
90	SBF402	Lactobacillus reuteri	Infant feces	1.27	9.55	++	++
91	SBF417	Lactobacillus reuteri	Infant feces	0.53	7.07	+++	+
92	SBF418	Lactobacillus reuteri	Infant feces	5.69	11.51	++	+
93	SBF419	Lactobacillus reuteri	Infant feces	0.32	5.36	+++	+
94	SBF420	Lactobacillus reuteri	Infant feces	0.29	6.19	+++	+
95	SBF447	Lactobacillus reuteri	Infant feces	0.66	6.86	+++	+
96	MBF301	Lactobacillus reuteri	Infant feces	0.99	7.02	+++	+
97	MBF302	Lactobacillus reuteri	Infant feces	0.25	5.39	+++	+
98	MBF303	Lactobacillus reuteri	Infant feces	0.47	5.20	+++	+
99	MBF304	Lactobacillus reuteri	Infant feces	0.41	4.18	+++	+
100	MBF307	Lactobacillus reuteri	Infant feces	12.83	17.83	+	+
101	MBF312	Lactobacillus reuteri	Infant feces	1.38	7.84	+++	+
102	MBF326	Lactobacillus reuteri	Infant feces	0.29	5.54	+++	+
103	MBF327	Lactobacillus reuteri	Infant feces	7.63	13.25	++	+
104	MBF329	Lactobacillus reuteri	Infant feces	12.96	17.58	+	+
105	MBF335	Lactobacillus reuteri	Infant feces	0.26	4.86	+++	+
106	MBF342	Lactobacillus reuteri	Infant feces	0.25	4.87	+++	+
107	MBF344	Lactobacillus reuteri	Infant feces	0.27	5.55	+++	+
108	MBF351	Lactobacillus reuteri	Infant feces	0.27	5.49	+++	+
109	MBF355	Lactobacillus reuteri	Infant feces	0.25	4.77	+++	+
110	MBF356	Lactobacillus reuteri	Infant feces	0.28	4.76	+++	+
111	MBF358	Lactobacillus reuteri	Infant feces	0.49	5.88	+++	+
112	MBF359	Lactobacillus reuteri	Infant feces	0.62	6.25	+++	+
113	MBF483	Lactobacillus reuteri	Infant feces	2.82	9.16	++	+
114	MBF484	Lactobacillus reuteri	Infant feces	0.26	4.76	+++	+
115	MBF496	Lactobacillus reuteri	Infant feces	0.24	5.71	+++	++
116	MBF497	Lactobacillus reuteri	Infant feces	0.27	4.88	+++	+
117	MBF498	Lactobacillus reuteri	Infant feces	0.23	4.64	+++	+
118	MBF4112	Lactobacillus reuteri	Infant feces	0.67	7.28	+++	+
119	MBF4116	Lactobacillus reuteri	Infant feces	0.24	4.23	+++	+
120	NBF306	Lactobacillus reuteri	Infant feces	0.27	4.40	+++	+
121	NBF307	Lactobacillus reuteri	Infant feces	0.66	7.74	++	+
122	NBF308	Lactobacillus reuteri	Infant feces	0.76	8.32	++	+
123	NBF309	Lactobacillus reuteri	Infant feces	0.90	7.88	++	+
124	NBF310	Lactobacillus reuteri	Infant feces	0.57	7.17	++	+
125	NBF317	Lactobacillus reuteri	Infant feces	12.66	18.11	+	+
126	NBF319	Lactobacillus reuteri	Infant feces	5.73	12.01	+	+
127	NBF320	Lactobacillus reuteri	Infant feces	8.06	13.97	+	+
128	NBF331	Lactobacillus reuteri	Infant feces	0.66	7.71	+++	+
129	NBF342	Lactobacillus reuteri	Infant feces	1.80	8.32	++	+
130	NBF344	Lactobacillus reuteri	Infant feces	2.10	8.96	++	+
131	NBF347	Lactobacillus reuteri	Infant feces	0.27	7.13	++	+
132	NBF409	Lactobacillus reuteri	Infant feces	0.09	5.73	+++	+
133	NBF430	Lactobacillus reuteri	Infant feces	0.10	5.80	+++	+
134	NBF456	Lactobacillus reuteri	Infant feces	0.10	5.50	+++	+
135	NBF474	Lactobacillus reuteri	Infant feces	0.66	9.50	++	++

^a1,3-PDO production:+++; > 12 g/L,++; > 7 g/L,+; > 0 g/L, –; not detected.
^bReuterin production:+++; > 0.5 at 560 nm,++; > 0.3 at 560 nm,+; > 0.1 at 560 nm, –; not detected.

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Table 1 Isolation of Lactobacillus reuteri strains, and determination of 1,3-PDO and reuterin production. 1,3-PDO and reuterin production were determined in Lactobacillus reuteri isolates using HPLC and Colorimetric method, respectively.

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