Figure 4
From: Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice
Vacuolation in the proximal tubules of WT-HFD and KO-HFD mice. (A) Toluidine blue staining (200 ×, scale bar: 50 µm) and (B) electron microscopy of the proximal tubules (1200 ×, scale bar: 5 µm). Aggregation with strong toluidine blue staining is observed in some proximal tubules in the WT-HFD group, whereas the aggregation is rarely observed in the KO-HFD group. Electron-dense multilamellar whirl structures are present in the cytoplasm of tubules in both groups. The accumulation is more extensive in the WT-HFD group than in the KO-HFD group. (C) Immunohistochemical staining for LAMP1 in the proximal tubules of the WT-HFD group shows that LAMP1 surrounds vacuolar membranes (200 × , scale bar: 50 µm).
