Table 2 Summary of cross reactivity study.

From: Development, evaluation of the PNA RT-LAMP assay for rapid molecular detection of SARS-CoV-2

Microorganisms

Hit rate (#detected/#tested)

Result

A well

B well

ORF1ab

RNase P

N

RNase P

1

Human coronavirus 229E

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

2

Human coronavirus OC43

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

3

Human coronavirus HKU1

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

4

Human coronavirus NL63

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

5

SARS-coronavirus

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

6

MERS-coronavirus

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

7

Adenovirus type 1

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

8

Adenovirus type 2

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

9

Adenovirus type 3

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

10

Human Metapneumovirus

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

11

Parainfluenza virus 1

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

12

Parainfluenza virus 2

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

13

Parainfluenza virus 3

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

14

Parainfluenza virus 4

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

15

Influenza A (H3N2)

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

16

Influenza A (H1N1)

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

17

Influenza B

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

18

Enterovirus

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

19

Respiratory syncytial virus

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

20

Rhinovirus 1

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

21

Rhinovirus 14

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

22

Rhinovirus 7

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

23

Chlamydia pneumoniae

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

24

Haemophilus influenzae

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

25

Legionella pneumophila

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

26

Mycobacterium tuberculosis

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

27

Streptococcus pneumoniae

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

28

Streptococcus pyogenes

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

29

Bordetella pertussis

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

30

Mycoplasma pneumoniae

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

31

Candida albicans

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

32

Pseudomonas aeruginosa

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

33

Staphylococcus epidermis

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

34

Streptococcus salivarius

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

35

Staphylococcus aureus

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

36

Human nasal wash

0% (0/3)

100% (3/3)

0% (0/3)

100% (3/3)

Negative

  1. RNA extracts from all tested microorganisms as well as direct human nasal wash were confirmed to not cross-react with PNA RT-LAMP assay.