Figure 3
From: Enhanced super-resolution microscopy by extreme value based emitter recovery
The comparison of different emitter recovery approaches using simulated dataset with various emitter size, emitter intensity and heterogeneous background. (a) The raw image (a1), the recovered emitters from the ground truth (TRUE) (a2), and using EVER (a3), MED (a4) and RB (a5). (b) The corresponding reconstructed super-resolution images using ThunderSTORM without additional processing (b1) and after emitter recovery using the ground truth (b2), EVER (b3), MED (b4) and RB (b5). Green arrows indicate the image artifact. (c) The corresponding intensity profiles (solid lines) from the regions between the blue bracket in (a1–a5) and the black dashed line (TRUE-BG) represents the profile from the ground-truth background. The black arrow indicates the erroneously recovered emitter signals by RB coming from the residual background. (d) The scatter plot of the localized emitters from the raw image without any processing (magenta) and with emitter recovery by the ground truth (black), EVER (red), MED (green) and RB (blue). Emitter localization was performed by ThunderSTORM. (e1–e3) The zoomed distribution of localized emitters in (c).
