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Figure 1

From: Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway

Figure 1

Isolation of potential CaSR interactors. (A) Co-immunoprecipitation experiments using anti-FLAG antibody on total cell extracts from HEK293 followed by western blot analysis with anti-CaSR antibody and anti-GAPDH (for total) was carried out in triplicates (one representative blot is shown). (B) Qualitative analysis of CaSR expression as normalized to the GAPDH in A (band intensity of CaSR divided by band intensity of GAPDH). (C) Venn diagram representing the number of proteins detected for each of the conditions. (D,E) Volcano plot of CaSR interactors (n = 3) enriched in 4 mM Ca2+ ((D), brown dots in right upper quadrant) and 2 mM EGTA perturbation (E, blue dots in upper right quadrant) in pairwise experiments versus negative control (without CaSR expression); n = 3. A total of 623 proteins are plotted (D,E) X-axis depicts enrichment in CaSR transfected (positive control) as compared to negative control. Y-axis depicts significance with p-value.

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