Figure 2
From: Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice

Inflammatory cytokines in the mouse colon after treatment with DSS and administration of TFA and PAT. (A–F) mRNA quantification of proinflammatory cytokines (TNF-α, IL-6, IFN-γ, IL-18 and IL-17a) and chemokines CCL2 using real-time RT–PCR. (G–I) Determination of inflammatory cytokine protein production using ELISA. The serum samples were collected on the 15th day. The data present the mean ± S.E.M. and n = 5/group. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.0001.