Figure 7
From: Total flavone of Abelmoschus Manihot improves colitis by promoting the growth of Akkermansia in mice
AKK ameliorated the symptoms of DSS-induced colitis in mice. (A) Daily bodyweight changes from day 1 to 14. (B) DAI score from day 1 to 14. *P < 0.05, compared with the DSS group; #P < 0.05, CON versus DSS group. (C) The lengths of colon. Data are presented as the mean ± S.E.M. and n = 6–8/group. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.0001. (D) Macroscopic appearances of colon tissues. (E) Histological changes. (F) mRNA quantification of proinflammatory cytokines (TNF-α, IL-6, IL-1β) using real-time RT–PCR. (G) Determination of protein production of inflammatory cytokines using ELISA. The data present the mean ± S.E.M. Asterisks denote significance versus DSS group by one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001). (H) The number of colonic MUC2-, KLF4-, and ZO-1-positive cells in DSS-treated mice. Representative images (× 200 magnification, scale bar 50 μm). (I–K) mRNA quantification of MUC2, KLF4 and ZO-1.
