Figure 2
From: Quantum chemical insight into the effects of the local electron environment on T2*-based MRI
T2* mapping detects Fenton chemistry. (A) Measurement of H2O2 using UV–Vis spectroscopy (240 nm; ε = 46.3 mol−1 cm−1; 1 cm pathlength) following 15 min incubation of 1.5 mM FAS ((NH4)2Fe(SO4)2 6H2O) with 100 mM H2O2 for 15 min in double-distilled H2O. (B) Measurement of Fe2+ following 1:15 dilution in 5 mM ferrozine using UV–Vis spectroscopy (562 nm; ε = 27,900 mol−1 cm−1; 1 cm pathlength). (C) Representative T2* map of 1:15 dilution in 1% agarose gel. (D) Mean T2* relaxation times quantified using Slicer3D software by generation a 1 mm ROI for each phantom and calculating the mean T2* relaxation time using the data quantification package within the software. Error bars represent SD of n = 3 biological replicates. Statistical analysis was performed using a one-way ANOVA test with statistical significance defined as a false positivity rate less than 5% (*p < 0.05).
