Table 1 Comparison of recent passive-driven approach of nucleic acid amplification microfluidic device.
Technology | Amplification technique | Material used | Sealed method | Auxiliaries involved | Characteristic of assay | References |
|---|---|---|---|---|---|---|
Centrifugal Microfluidic Platform | PCR | Polymethyl methacrylate (PMMA) | Mineral oil injection | Rotary motor | HBV detection from whole blood based on centrifugal microfluidic platform. Pipette injection with mineral oil | |
Smartphone-based multiplex test | LAMP | Sillicon wafer with SiO2 film as microfluidic channel | Sealed with double side adhesive layer and covered with glass on top | Pipette | Fluorescence images of LAMP amplification reaction were taken by a smartphone | |
SlipChip/ sp-SlipChip | LAMP | Two layer of glass plate | Lubricating oil were placed between two plates | Pipette | Reagent self-partition into individual droplets driven by surface tension-driven with slipping the two plate | |
Self-driven microfluidic chip | LAMP | PDMS with hydrophilic UV cured glue | Closed the channel with air-control channel via vacuum system | Pipette, electro-magnetic valves, compact vacuum pump | Hydrophilic surface drives the LAMP reagent flow into the channel | |
MF-LAMP | LAMP | PDMS, Glass | Mineral oil injection and outer channel surrounded | Pipette | Capillary channel controls the fluid exchange, partition, sealed process avoiding water loss in the device | This study |
